Pdf file formats commonly used in mass spectrometry. Lecture 1 fundamentals of protein chemistry and mass spectrometry arthur moseley arthur. Mass spectroscopy mass spectroscopy is a technique causing the formation of the gaseous ions with or without fragmentation. Thomson discovers the electron and determines its mz nobel prize in 1906 1912 j. Goldstein discovers anode rays in a gas discharge tube. Chapter 2 fragmentation and interpretation of spectra 2.
When students ask what books to read on mass spectrometry, there are various excellent monographs, but the ideal textbook still seemed to be missing at least in my opinion. If the mass spectrometer has a mass range of up to 4,000, how can you analyse high molecular weight proteins. Note that modern spectrometers actually indicate that neon has a third isotope. It is critical that you develop a working knowledge of how each of these techniques provides structural information about organic molecules. Mass spectrometry ms mass spectrometry is a method that enables precise measurement of molecular weight of a broad spectrum of substances. The modules are highly adaptable and can be applied to young children from the age of 6.
The lecture notes for this course are available in a single file pdf 2. Lecture note on mass spectroscopy time of flight mass. Ms is a technique that allows the detection of compounds by separating ions by their unique mass mass tocharge ratios using a mass spectrometer. Lecture notes in eimass spectrometry by torben lund. The result of a mass spectrometry is a graph plotting mass per charge against relative abundance. The development of detectors and ionization techniques and their coupling with chromatography have broadened the range of combinations, including ionic compounds or macromolecules greatly 38. The mass spectrometer gives the mass to charge ratio mz, therefore the sample analyte must be an ion. For the analytical chemist, a mass spectrum is useful for two applications. How do you separate ions of different mass, so that you can detectquantify them. Single ion mass spectrometry at 100 ppt and beyond 191. These lecture notes were first drafted in dutch by peter van galen in 1992, when the only mass spectrometer in the faculty of science was the departments.
Data system mass detector analyzer vacuum system source inlet region more precisely mass spectrometry determines the mass of a molecule. Pdf on jun 7, 2017, teodor octavian nicolescu and others published interpretation of. The lecture notes files in the table are the slides shown in class. The mass spectrum allows an experienced analyst to identify the compound or. Asparagine and glutamine are amide derivatives of aspartate and glutamate, respectively.
The presented teaching modules introduce young children to mass spectrometry and give them the opportunity to experience a modern research laboratory. This is the first of a pair of videos intended to help senior school chemistry students with mass spectrometry. An introduction to mass spectrometry these pages have moved. Find materials for this course in the pages linked along the left. The leading scientist in this field was fred mclafferty who together with frantisek turecek has written a very. It can be used to find relative isotopic abundance, atomic and molecular mass, and the structure of a compound.
One may note that, the same as in all compounds with two bromine atoms. Masses in mass spectrometry the average mass of a molecule is obtained by summing the average atomic masses of the constituent elements. Thomson constructs the first mass spectrometerand sees spectra of o 2, n 2, co, co 2 and cocl 2. The contents of the solvent spray, the gas flow rate, the amount of applied voltage, the spray angle and the ion uptake angle, as well as the various distances in aligning the spray, sample and mass spectrometer are all variables which can be studied to achieve an optimal mass spectrum for a particular type of sample.
Anatomy of an irms lecture 6 mass analyzers 1 assigned reading. Where available, lecture notes or unit study guides are included for the following sessions and topics in the table below. By convention, m is the numerical value for the mass of the ion and z is the numerical value for the charge of the ion. This chapter provides a general introduction to ms, mainly from a functional point of view. Lecture note on mass spectroscopy free download as pdf file.
Most of these methods employ differential stable isotope labeling to create a specific mass tag that can be recognized by a mass spectrometer and at the same time provide the basis for quantification. Run times will depend on the salt concentration of the sample, size of the strip, and ph range. From the time of the second edition published in 2001 until now, much. It can be used to find relative isotopic abundance, atomic and molecular mass, and the structure of a compound the result of a mass spectrometry is a graph plotting mass per charge against relative abundance. Mass spectrometry is a destructive method used to measure molecular weight and provide. Get private tutoring from anywhere in the world, via your computer, f. The ionized molecule often fragments into smaller ionsradicals. Mass spectrometry dont call it spectroscopy absorption of electromagnetic radiation electron impact mass spectrometry 70 ev 1614 kcalmol contrast with energy from ir 110 kcalmol or nmr 0. Lncs 627 single ion mass spectrometry at 100 ppt and.
Fast atom bombardment and secondary ion mass spectrometry. Chapter 2 protein structure 31 side chains with polar but uncharged groups six amino acids have side chains with polar groups figure 2. The ph gradient can be over a single ph unit or up to 10 ph units. Fluorescence emission from excited electronic singlet states phosphorescence emission from excited electronic triplet states. A plot of relative abundance of ions vs mz of all charged particles is the mass spectrum. Lecture notes electron microprobe analysis by wavelength. Scientific instrument services, sis, usa e electrospray ionization mass spectrometry an introduction format. Mass spectrometry m e m e molecular ion peak is at the right end of the mass spectrum xaxis. It is used for determining masses of particles, for determining the elemental composition of a sample or molecule. Mass spectrometry is a gas phase technique the sample must be vaporized. In physics and chemistry spectroscopy is an often used anlytical technique to identify chemical compounds through the spectrum emitted from or absorbed by them. Chemistry 344 spectroscopy and spectrometry uwmadison. There are five general types of mass analyzers that can be used for the separation of ions in a mass spectrometry.
He observes negative ions, multiply charged ions and identifies isotopes of 20ne and 22ne. Serine, threonine, and tyrosine have side chains with hydroxyl oh groups. Fluorescence emission from excited electronic singlet states phosphorescence. A mass spectrometer generates multiple ions from the. Application notes over 80 articles on thermal desorption, ms direct probe, and simion, including papers we presented at pittcon, asms, and eas. Objects atoms or groups of atoms of different masses may be detected due. This is a lecture and the second video takes you through past paper questions. Introduction mass spectrometry ms is an analytical technique that measures the mass tocharge particles. Lecture 1 introduction, history, scope and terminology of mass spectrometry 1. Multiply charged myoglobin ions from esi 0 10 20 30 40 50 60 70 80 90 100 1060.
The mass to charge ratio mz is used to describe ions observed in mass spectrometry. Our notes are easytofollow, handwritten chemistry pdf notes that combine your book and chemistry notes into one, simple, easytounderstand format. As the studied substance has to be intact in gas phase, the use of mass spectrometry for protein analysis but also of polysaccharides and oligonucleotides. Excitation induces emission of light from the sample usually of different frequency. The proportionality of signal intensity and the amount of analyte allow the use of mass spectrometry in quantitative assays. The magnetic field induces a circular course to electrons. Lecture 1 introduction, history, scope and terminology of. Mass spectrometry is a process by which the atomic mass of atoms or molecules is determined. Ea elemental analysis ms mass spectrometry ir infrared spectroscopy nmr nuclear magnetic resonance. Mass spectrometry ionisation techniques how do we handle different types of sample. Mass spectrometry ms has been wi dely used in forensic science in the identification of compounds, particularly illicit drugs. Electrospray mass spectra interpreting electrospray mass spectra. One theory is that as the liquid leaves the nozzle, the electric field induces a.
Magnetic electrostatic double sector mass analyzer 4. Mass spectrometers see the isotope peaks provided the resolution is high. Overview of a mass analyzer michigan state university. Run times vary from 26 hours for tube gels to 624 hours for the ph strips. We have both general chemistry notes and organic chemistry notes. Chemistry notes download handwritten pdf chemistry notes. Lecture 1 fundamentals of protein chemistry and mass.
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